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Objective@#To study the effect and mechanism of the extract from grub on the CD40 of microglia cells in rabbit model of central retinal vein occlusion.@*Methods@#The 60 color rabbits were randomly divided into the blank group, model group, Xueshuantong group and sputum group. The Xueshuantong group was intragastrically administered with Xueshuantong tablets suspension 5 mg/ml, the sputum group was given gavage extract 1 g/ml, and the blank group and the model group were intragastrically administered with normal saline once daily. Except for the blank group, the other groups of rabbits were modeled by argon laser irradiation of the retinal vein trunk, and the fundus photography and FFA examination were performed immediately after modeling and at 1, 14 and 28 days after modeling, respectively, at 1, 3, 7, 14 and 28 day, the expression of CD40 in the optic nerve of rabbits was observed by immunohistochemical staining.@*Results@#The FFA results showed that the veins were not filled at 1 day after model establishment, and some veins were not developed at 14 days, and there was no evidence of revascularization at 28 days. Part of the venous in the thrombus group and the sputum group was not filled. The venous filling time was significantly shorter than that in the model group at 14 days, and the venous filling time returned to the normal level at 28 days. The results of immunohistochemistry showed that the number of microglia increased significantly at 1 d in model group. The number of microglia reached the highest peak at 3 d, and the number of microglia decreased at 7 d, 14 d and 28 d. Compared to the model group, at the 3, 7, 14, 28 d, the integrated optical density of CD40 (3 d: 8 908.91 ± 96.30, 6 099.92 ± 273.44 vs. 10 436.4 ± 1 306.8; 7 d: 5 982.06 ± 483.37, 2 957.36 ± 424.19 vs. 8 798.12 ± 444.39; 14 d: 3 225.36 ± 468.88, 342.04 ± 64.56 vs. 5 356.74 ± 439.16; 28 d: 756.97 ± 80.17, 72.85 ± 11.06 vs. 4 215.27 ± 361.00) in the Xueshuantong group and sputum group significantly decreased (P<0.05).@*Conclusions@#The thrombus and sputum extract can inhibit the activation of microglia to varying degrees, and the sputum extract is moreinhibitory effect.
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Objective To study the effect and mechanism of the extract from grub on the CD40 of microglia cells in rabbit model of central retinal vein occlusion. Methods The 60 color rabbits were randomly divided into the blank group, model group, Xueshuantong group and sputum group. The Xueshuantong group was intragastrically administered with Xueshuantong tablets suspension 5 mg/ml, the sputum group was given gavage extract 1 g/ml, and the blank group and the model group were intragastrically administered with normal saline once daily. Except for the blank group, the other groups of rabbits were modeled by argon laser irradiation of the retinal vein trunk, and the fundus photography and FFA examination were performed immediately after modeling and at 1, 14 and 28 days after modeling, respectively, at 1, 3, 7, 14 and 28 day, the expression of CD40 in the optic nerve of rabbits was observed by immunohistochemical staining. Results The FFA results showed that the veins were not filled at 1 day after model establishment, and some veins were not developed at 14 days, and there was no evidence of revascularization at 28 days. Part of the venous in the thrombus group and the sputum group was not filled. The venous filling time was significantly shorter than that in the model group at 14 days, and the venous filling time returned to the normal level at 28 days. The results of immunohistochemistry showed that the number of microglia increased significantly at 1 d in model group. The number of microglia reached the highest peak at 3 d, and the number of microglia decreased at 7 d, 14 d and 28 d. Compared to the model group, at the 3, 7, 14, 28 d, the integrated optical density of CD40 (3 d: 8 908.91 ± 96.30, 6 099.92 ± 273.44 vs. 10 436.4 ± 1 306.8; 7 d: 5 982.06 ± 483.37, 2 957.36 ± 424.19 vs. 8 798.12 ± 444.39; 14 d:3 225.36 ± 468.88, 342.04 ± 64.56 vs. 5 356.74 ± 439.16; 28 d: 756.97 ± 80.17, 72.85 ± 11.06 vs. 4 215.27 ± 361.00) in the Xueshuantong group and sputum group significantly decreased ( P<0.05). Conclusions The thrombus and sputum extract can inhibit the activation of microglia to varying degrees, and the sputum extract is moreinhibitory effect.
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Objective: To observe the effect of Yiqi Mingmu Pill on the expression of BaxmRNA protein and caspase-3 mRNA in retina of RCS rats. Methods: Twenty-four RCS rats were randomly divided into three groups: blank group, model group, Yiqi Mingmu Pill group. In the blank group, there were 8 RCS (rdy+/+, p+/+) rats, with 4 males and 4 females, and they received intragastric administration of normal saline. The model group consisted of 8 RCS (rdy-/, p-/-) rats, with 4 males and 4 females, and they received intragastric administration of normal saline. Yiqi Mingmu Pills groupconsisted of 8 RCS (rdy-/, p-/-) rats, with 4 males and 4 females, respectively. They received intragastric administrationof suspension of Yiqi Mingmu Pills. After intragastric administration for 30 days, the structure of retina layers wasobserved by HE staining. BaxmRNA and Caspase-3 mRNA in retinal tissues of each group were measured by RT-qPCRand Western Blot. Results: HE staining showed that the retina thickness of rats in Yiqi Mingmu Pill group wassignificantly thicker than that of the model group. The retinal pigment epithelial band was partially visible, and part of theouter nuclear layer photoreceptor sensory ciliary layer was visible. The number of photoreceptor cell nuclei was higherthan that of the model. There were many groups. The outer layer, outer core layer, and the visual cone layer were clearerand thicker than the model group. RT-qPCR showed that the relative expression of Bax mRNA and Caspase-3 mRNA inYiqi Mingmu Pills group was significantly lower than the model group, and the difference was statistically significant (P＜0.01), WB test showed that the relative expression of Bax protein and Caspase-3 protein in Yiqi Mingmu Pill group wassignificantly lower than that in the model group, and the difference was statistically significant (P＜0.01) . Conclusion: YiqiMingmu Pill has protective effect on the ultrastructure of RP retina. Yiqi Mingmu Pills can reduce the apoptosis of retinalphotoreceptor cells by inhibiting the expression of BaxmRNA and Caspase-3 mRNA on the retina and protect the visualcells.
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Objective To observe the effects of electro-acupuncture (EA) on body weight, insulin resistance, hypothalamic agouti gene-related protein (AGRP) and neuropeptide Y (NPY) in diet induced obesity (DIO) rats; To discuss its mechanism for DIO.Methods Fifty SD male rats were randomly divided into low fat diet groups (LFD) and high fat diet group. After the DIO models were established, the successful model rats were randomly divided into model group, EA group and Orlistat (OLST) group. EA was applied to “Zusanli” (ST36) and “Quchi” (LI11) for 20 minutes. The treatment was done once a day for 28 days. OLST was treated with orlistat by gavage. LFD and model did not receive treatment. Body weight was recorded every day. FPG and FINS were detected. The expressions of AGRP and NPY were detected by RT-QPCR. Morphological changes of adipocyte and liver were examined by HE staining.Results The body weight, HOMA-IR, AGRP, NPY and diameter of adipocyte of EA group and OLST group were significantly lower than model group (P<0.05), difference between EA group and OLST group. The states of hepatic steatosis were improved in EA group and OLST group.Conclusion EA has the effects on weight loss by inhibiting the expressions of AGRP and NPY by improving IR.
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BACKGROUND:With the development of fixation materials and technology, scholars began to use artificial bone materials combined with pedicle screw fixation in the repair of spinal tuberculosis. OBJECTIVE: To summarize the characteristics of artificial bone materials combined with pedicle screw fixation in the repair of spinal tuberculosis. METHODS:The literatures about artificial bone graft materials in repair of spinal tuberculosis were retrieved from CNKI and PubMed database during 1985 to 2014 by computer. The keywords were “spinal tuberculosis, bone transplantation, internal fixation” in Chinese and English, respectively. RESULTS AND CONCLUSION:In the repair of spinal tuberculosis using artificial bone materials combined with pedicle screw fixation, autologous bone is the gold standard for bone grafting, presenting with no immune rejection. To avoid the occurrence of limited bone mass, pain and other complications, however, artificial bone materials have been extensively studied. Currently used artificial materials mainly include titanium and its aloys, hydroxyapatite composites and medical calcium sulfate, which al have good biocompatibility. But there are stil certain limitations and shortcomings.
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This study was aimed to evaluate the application of64-slice spiral CT in imaging anatomy of the normal optic canal. The optic canals of 100 healthy subjects were examined by 64-slice spiral CT,and the imaging measurement of the anatomy structures were performed by the multi-planar reconstruction. The longest and the shorzest walls of the optic canal were the medial wall and the inferior wall, respectively.Results indicate that the imaging anatomic measurement of the optic canal can be accurately and easily performed by means of multi-planar reconstruction of 64-slice spiral CT.